BIOSKETCH
Giandomenico obtained his bachelor’s and master’ degrees in Biological Sciences and Pathophysiology from the University of Messina in 2002. In the same year, he successfully passed the state exam, qualifying him to practice as a biologist. From 2004 to 2006, he worked as a Researcher Specialist in Transplantation Immunogenetics at the Tissue Typing Laboratory of the Transfusion Medicine Unit at the Cervello hospital in Palermo. At the same time, he underwent a training internship at the National Research Council (CNR) in Rome, focusing on the implementation of cross-match procedures in cytofluorimetry. He also obtained a post-graduated diploma in Microbiology and Virology from the University of Palermo. In 2010, he completed a two-year Level II Master’s Degree in Immunogenetics and Immunopathology, at the Department of Biopathology, University of Palermo. In 2008, he joined Ri.MED Foundation as a researcher associate. Later in 2014, he obtained a Ph.D in Molecular and Experimental Medicine from the University of Palermo. In November 2023, he completes his master’s degree in Immuno-Oncology at the NIBIT Foundation in Siena, Italy. Currently, he is a Senior Specialist in flow cytometry at the Regenerative Medicine and Biomedical Technologies Unit of the Ri.MED Foundation, which is located at the Mediterranean Institute for Transplantations and Advanced Therapies (IRCCS ISMETT). Thanks to his versatile role in the FACS Core Facility, Mimmo has contributed to the various research projects of both Ri.MED and IRCCS ISMETT. His scientific impact is evident with 26 peer-reviewed publications, 638 citations, and a H-index of 14.
https://orcid.org/0000-0002-6957-9861
Scientific Activities
Mimmo’s activity focuses on the cytofluorimetric characterization and sorting of various cell types, including hematopoietic cells, CTLs, dendritic cells, T, B and NK lymphocytes, as well as human mesenchymal stromal/stem cells (MSCs) from placenta, umbilical cord, fetal and adult dermis. For this purpose, he utilizes the following methodologies:
- 16-color multiparametric immunophenotyped (project “Adoptive cellular immunotherapy of post-transplant viral infections”)
- Apoptosis and cell cycle assays on HepG2 and K562 cell lines to test anti-blastic drugs.
- External UK NEQAS quality controls for CD34+ count and immunophenotype of lymphocyte subpopulations
- Diagnosis of Paroxysmal Nocturnal Hemoglobinuria (EPN or PNH)
- Cytofluorimetric characterization of human keratinocytes and fibroblasts
- Analysis of regulatory T cells, Th1, Th2, Th9, Th17, Th22, cell cycle assessment and apoptosis by Annexin V using flow cytometry
- Quantification of growth factors and cytokines secreted by MSCs using Luminex technology
- Absolute count of CD34+ hematopoietic stem cells
- Analysis and sorting of MSCs