Project title:Translating protection of cell-surface receptor LRP1 into potential disease-modifying therapies for osteoarthritis
Start/end date: may 2023 – may 2025
Funding Agency/Programme: Versus Arthritis – Bridging fellowship 2023, on behalf of the project Coordinator University of Liverpool (United Kingdom).
Partnership: University of Liverpool – UK (Coordinatore); Collaboratori: University of Surrey; Fondazione Ri.MED.
Objective: using high-resolution proteomics to identify binding partners of the low-density-liporptoein receptor related protein 1 (LRP-1) in osteoarthritic cartilage. In brief, proteins will be purified from osteoarthritic and normal cartilage, and then applied to an agarose resin coupled with LRP-1 previously purified from placenta. Proteins binding to LRP-1 will be eluted, digested by FASP and analysed by LC-MS/MS.
Ri.MED Principal Investigator: Simone Dario Scilabra